Cell Biology

Cell biology provides high throughput custom designed cell based assays for molecular targets, screening strategies and cell based proof of concept experiments for drug molecules.

Cell culture: Development and optimization for primary culture cell lines (Rat hepatocytes and macrophages), recombinant cell line, insect cell culture, cancer and immortalized cell lines

Cell line development: Development of stable and transient cell lines by using transfection methods for screening the drug molecules.

Cytotoxic/Cell proliferation assays: Evaluating the cytotoxic property of the test item using assays such as MTT, SRB, 3H Thymidine incorporation, Alamar blue reduction, CyQUANT etc.

Cell Based ELISA: Development, validation and high throughput screening of compounds for cellular target

Apoptosis assays: Screening assays for detection and measurement of drug induced apoptosis

Immunocytochemistry: Development of immunological staining method on tissues and cells to localise the cellular target like proliferation markers, apoptosis (TUNEL assay) and target modulations.