Molecular Biology

Various assays are developed using molecular biology tools to screen and identify molecular targets.

Gene cloning: Gene encoding molecular targets are cloned into suitable vector and high level expression of target protein using bacterial, bacculovirus (insect cells) and mammalian systems. The purified protein and enzymes are used in biological assays and can also be supplied to our clients.

cDNA library: Generating the cDNA library for the genes of interest from various tissues and cell cultures. These libraries are cloned and used in bioassays.

Reporter based assays: Constructing the reporter gene into the luciferase vector and developing the bioluminescent assay for molecular targets.

Site directed mutagenesis: Mutation generation in the particular site of  DNA and used in the mutational analysis of the proteins.

Blotting techniques: Western, northern and dot blotting experiments are performed to analyse the gene and protein expressions to identify a drug’s mechanism of action.

Kinase assays: Development of radiometric kinase assays to identify kinase inhibitors. Kinase profiler assays are performed to identify the specificity of the test item.

Receptor binding assay: Development, validation and screening of drug affinity and selectivity for various transmembrane receptors by using radiometric assays.