Molecular Biology
Many clients have utilized Anthem's custom molecular biology service that ranges from cloning and expression, to protein purification. Our scientists can help you custom design solutions based on our expertise, and walk you through the various steps. Our dedicated project managers will communicate in a timely manner the progress of your project. We have successfully executed many projects that encompass cDNA synthesis, site directed mutagenesis, cloning, expression and protein purifications in an economically viable manner.GENE SYNTHESIS AND CLONING
If you have a cDNA, a promoter, or genomic DNA to be cloned into a vector for any desired
downstream application, we have the capability to execute your project. We can clone your
DNA of interest into your vector of choice. We can take on projects from cDNA sequence to
protein promoter to reporter systems, site-directed mutagenesis, cDNA and genomic DNA
libraries.
EXPRESSION ANALYSIS
We have experienced scientists to take care of your mRNA, miRNA and protein isolation
needs from various cell types and tissue samples. Messenger RNA and miRNA are
quantitatively analyzed by real time RT-PCR, and protein expression analysis is performed
using Western blotting, or ELISA.
PROTEIN EXPRESSION AND PRODUCTION
Bacterial
Anthem Biosciences offers comprehensive services in cloning, expression, and purification of peptides and proteins in E-coli. We have purified therapeutic proteins, growth hormones, industrial enzymes, and viral proteins in bacteria for our clients. We also have the expertise to target recombinant proteins to specific compartments of E-coli such as cytoplasm, periplasm, cell membrane, and external media.Yeast
Anthem scientists have significant experience in providing services using yeast expression systems like Pichia pastoris. Anthem's rich experience in bio-process optimization has been employed by several companies.Mammalian
Transient expression of proteins using plasmid transfections, and expression in custom stable engineered cell lines using lentiviral vectors can be performed at Anthem Biosciences. We offer guaranteed high level expression of single or more heterologous proteins in CHO and 293 cells. We can also express proteins in other cell lines based on our client needs taking advantage of the broad tropism of lentiviral transduction.Baculoviral
We have used this system to express proteins for some of our clients.We have optimized media and growth conditions for all the above expression systems to increase the yield for our clients.
VIROLOGY
At Anthem Biosciences, we offer basic virology laboratory services (BSL2 viruses) and can customize protocols to suit the specific needs of our clients.
The list of virology services currently available at Anthem are:
- Isolation of virus from patient samples
- Propagation of human & animal viruses
- Isolation of viral protein, RNA & DNA from infected cells
- Plaque Assay and TCID50 calculations
- Detection of virus in infected cell lines (PCR and QPCR services)
- Virus expression systems Concentrating virus and titering the concentrated virus to check for virus viability in the stock
- Immunoassay services such as plaque reduction neutralization assays, DNA transfection and protein detection (ELISA and Western Blot)
PROTEIN PRODUCTION
Anthem Biosciences has the capability to produce and purify recombinant proteins for various research and industrial applications.
We offer the following chromatographic protein purification/analysis techniques:
We offer the following chromatographic protein purification/analysis techniques:
- Ion-exchange chromatography
- Metal Affinity chromatography
- Hydrophobic interaction chromatography
- Affinity chromatography
- Size-exclusion chromatography
- RP-HPLC
Integrated Discovery
Facilities