Antibody Generation
Unlocking Therapeutic and Diagnostic Potential
Antibodies are critical tools in modern medicine, serving as targeted therapeutics, diagnostic reagents and research tools. The generation of high-quality, specific antibodies is foundational to biologics development.
Traditional Antibody Generation Methods
Polyclonal Antibody Production
- Immunization of animals (rabbits, larger mammals) followed by serum collection and purification yields polyclonal antibodies with broad epitope recognition. While effective, these antibodies can suffer from batch variability and limited characterization.
Hybridoma Technology
- The gold standard for monoclonal antibody (mAb) production, hybridoma technology involves immunizing animals, isolating antibody-secreting B cells and fusing them with immortal myeloma cells. Cloning ensures stable, monospecific antibody production. Anthem supports the entire hybridoma workflow, including screening and scale-up.
Advanced Antibody Discovery Technologies
Single B Cell Cloning
This innovative method isolates antigen-specific B cells directly from immunized hosts, enabling rapid generation of native monoclonal antibodies with natural post-translational modifications. Techniques like fluorescence-activated cell sorting (FACS) and optofluidic platforms accelerate discovery timelines significantly.
Phage Display and Other Display Technologies
In vitro display platforms generate vast antibody libraries, allowing iterative selection (“panning”) of high-affinity binders. These methods can be combined with immunization strategies to enhance antibody diversity and specificity.
Hyperimmune and Transgenic Animal Models
Specialized mouse strains with enhanced immune responses (e.g., DiversimAb™) enable generation of antibodies against challenging targets, including GPCRs and ion channels.
Custom Antibody Services at Anthem
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Immunogen/ Antigen Design We design and produce recombinant proteins, peptides or conjugates optimized to elicit
strong immune responses. -
Hybridoma Generation & Screening End-to-end development from animal immunization to clone selection and antibody characterization.
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Scale-Up & GMP Production Large-scale antibody manufacturing under GMP conditions, including purification and quality
control, supporting clinical and commercial needs.
Comprehensive
Expertise
State-of-the-Art Technology
Regulatory
Compliance
