Group Companies

Top quality service, timely delivery and proactive communication

Group Companies

One stop solution for integrated drug discovery, process development and scale-up

Group Companies

Anthem works closely with clients on IP related information

Group Companies

The only CRO in the country with Microbiology division that provides antiinfectives screening services

Group Companies

Proven Track Record for Hit-Identification, Hit to Lead and lead Optimization

Group Companies

Highly qualified scientific team with diverse experience

Group Companies

Cloning, expression and purification of recombinant proteins from both bacterial and mammalian host systems.

Invivo Pharmacology

Anthem's in vivo Biology department has the infrastructure and proven expertise to conduct efficacy studies in various disease models in several therapeutic areas including oncology, metabolic disorders, inflammation and infection. Anthem offers customizable pharmacology services following custom designed protocols to support your drug discovery needs. Anthem has a state-of- the art vivarium equipped to house immuno-compromised animals, genetic models, rodents and rabbits. All experimental procedures involving animals are approved by the Institutional Animal Ethics Committee (IAEC) under the supervision of Committee For The Purpose Of Control and Supervision on Experiments on Animals (CPCSEA). Establishment and validation of disease models are an ongoing effort with several models being added. Please enquire for further details.


Human xenograft tumor models in immunocompromised mice
  • Validated xenograft models
  • Strains: Nude and SCID mice
  • Maximum tolerated dose determination (MTD)
  • Evaluation of mono and combination therapy
  • Cell Lines: Prostate (PC3) ; Breast (MCF7); Colon (HT-29); Ovary (SK-OV-3); Lung (A549); Pancreas (PANC-1).
Efficacy evaluation combined with
  • Hematology analysis
  • PK sampling and analysis
  • Histopathology or tissue sampling
  • Biomarker evaluation
  • Correlation of in vivo efficacy with target inhibition by tissue WB
Models under validation
  • Syngeneic tumor model
  • Metastasis models
  • Matrigel plug angiogenesis model


Diabetes/Obesity model
Genetic models of diabetes/obesity
  • db/db, ob/ob mice, Zucker fa/fa and ZDF rat High Fat Diet Induced obesity and diabetes in mouse/rat
  • Type I diabetes: STZ induced and NOD mouse
  • Glucose tolerance (OGTT, IPGTT)
  • Insulin tolerance test (IPITT)
  • High fat induced dyslipidemia in rat
  • Transgenic mice
  • Lipoprotein measurement
  • Lipid tolerance (IVLTT)
  • Triton induced TG secretion
Biomarker analysis
  • Blood chemistry: Glucose, triglyceride & cholesterol, lipoproteins, Lactate NEFA in plasma.
  • Insulin, Leptin, adiponectin and GLP-1 measurement.
  • Lipoproteins, Apo A-I, Apo B
  • Gene/Protein expression analysis in cells & ex-vivo (qPCR, ELISA, WB)


Inflammation and Arthritis
  • Carrageenan induced paw edema
  • Collagen-induced Arthritis (CIA) in DBA1 mice/ Lewis rat
  • Adjuvant-induced Arthritis (CIA) in DBA1 mice/ Lewis rat
  • LPS induced sepsis
  • Immunomodulatory studies: Ex vivo study in mouse splenocytes
  • DSS induced colitis in mice (Disease activity index, Colon histopathology)
Biomarker analysis
  • Inflammatory markers.
  • Cytokine analysis in plasma/tissue (TNF-α, IL-6, IL-1).
  • Histopathology and Immunohistochemistry
Models under validation
  • Nociceptive pain
  • Acetic Acid Writhing Test in Mice
  • Formalin Induced Nociception
  • Hot Plate model
  • Tail flick model


Murine experimental infection models
I. Systemic Infection Models
  • Efficacy - Outcome model
  • Species/strain: CD-1 mice
  • Challenge organism: E. coli ATCC 25922 / S. aureus ATCC 29213 / P. aeruginosa ATCC 27853
  • ED50 by Reed and Muench method
II. Organ Burden Models
  • Thigh infection: E. coli ATCC 25922 / S. aureus ATCC 29213
  • Transurethral Pyelonephritis model: E. coli ATCC 25922/ K. pneumoniae ATCC 700603
  • Acute Lung infection: S. pneumoniae ATCC 49619 Intranasal route of infection
  • Species/strain: Mouse: CD-1, Swiss Albino
  • Endpoint - CFU enumeration from tissue/ log reduction
  • PK-PD correlation
Integrated Discovery
Email ID
Contact Number