Anthem
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Anthem
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Anthem
Group Companies
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Anthem
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Group Companies
Anthem
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Anthem
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Anthem
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Anti Infectives
Our microbiologists are well trained scientists conducting both standard and customizable in vitro and in vivo anti-infective studies for our clients in our well equipped facility. We also have the capability to perform radio activity based studies. Anthem has a vivarium equipped to carry out anti-infective studies.In vitro MICROBIOLOGY
GENETIC TOXICITY STUDIES
- Microbial characterization (biochemical and antibiotic resistance)
- Primary screening MIC-CLSI broth dilution (antibacterial and antifungal, MIC50, MIC90, MIC with variable elements (serum, albumin, inoculum, temperature effect) and WHONET analysis
- Resistance development
- Secondary screening Kill kinetics (TK), Post-antibiotic effect (PAE)
- Tertiary screening Mutation prevention concentration and frequency
- Microbial biofilm susceptibility
- Efflux pump inhibitor screen (ethidium bromide accumulation)
- Drug combination study (FIC index, synergism/antagonism /indifference /additive effect)
- Hemolytic activity
- Bacteriophage isolation and characterization
- Mechanism of action
- S-35 methionine incorporation (for de novo protein synthesis)
- Bacterial-mammalian host interaction assay (binding and invasion)
In vivo MICROBIOLOGY
Systemic Infection Models
- Efficacy - Outcome model
- Species/strain: CD-1 mice
- Challenge organism: E. coli ATCC 25922 / S. aureus ATCC 29213 / P. aeruginosa ATCC 27853
- ED50 by Reed and Muench method
Organ Burden Models
- Thigh infection: E. coli ATCC 25922 / S. aureus ATCC 29213, i.m route
- Acute Lung infection: S. pneumoniae ATCC 49619 Intranasal route of infection
- Species/strain: Mouse: CD-1, Swiss Albino
- Endpoint - CFU enumeration from tissue/ log reduction
- PK-PD correlation
Integrated Discovery
Facilities
